药物合成生物学

[1] 国家重点研发计划项目子课题：以提高水溶性为导向的硫肽类化合物衍生物定向生物合成                                               在研

[2] 国家自然科学基金面上项目：以改善结构稳定性为导向的诺卡沙星衍生物定向生物合成                                                 在研

[3]  “重大新药创制”国家科技重大专项子课题：基于生物合成途径改造的关键技术和HXSW-101临床前研究                                已结题

[1] Du, Y.L. ^#, Xia, Y. ^#, Wu, L.R., Chen, L., Rong, J.L., Fan, J.T., Chen, Y. J., Wu, X. R*. Selective biosynthesis of a rhamnosyl nosiheptide by a novel bacterial rhamnosyltransferase. Microb. Biotechnol. 2024, 00:e14412.

[2] Zhao, L. ^#, Xu, Y. C. ^#, Chen, M. T., Wu, L.R., Li, M., Lu, Y. Y., Lu, M. L., Chen, Y. J., Wu, X. R*. Design of a chimeric glycosyltransferase OleD for the site-specific O-monoglycosylation of 3- hydroxypyridine in nosiheptide. Microb. Biotechnol. 2023, 16(10): 1971-1984.                        

[3] Zhang, B. ^#, Jin, W.Z. ^#, Zhang, Y.Y., Dai, Y., Li, H., Sun, Y,Z., Wu, X.R.*, Luo J.*, Chen, Y.J.* A type I/type III PKS hybrid generates Cinnamomycin A-D. Org. Lett., 2023, 25: 2560-2564.                            

[4] Su, Y.X., Li, Z.L., Zhao, Y.H., Chen, Y.J., Luo, C., Wu, X.R.* Enzymatic hydrolyzation of mogrosides in Luo Han Guo extract by NKA-adsorbed snailase improves its sensory profile. Food Chem., 2022, 390: 133205.

[5] Xu, Y.C.^#, Liu, S.Q. ^#, Bian L.Y. ^#, Li Z.L., Luo C., Chen, Y.J.*, Wu, X.R.* Engineering of a UDP-Glycosyltransferase for the Efficient Whole-Cell Biosynthesis of Siamenoside I in Escherichia coli. J. Agric. Food. Chem., 2022, 70(5): 1601-1609. doi: 10.1021/acs.jafc.1c07699.       

[6] Xu, Y.C.^#, Zhao, L.^#, Chen, L., Du, Y.L., Lu, Y.Y., Luo, C., Chen, Y.J. *, Wu, X.R.* Selective enzymatic α-1,6-monoglucosylation of mogroside IIIE for the bio-creation of α-siamenoside I, a potential high-intensity sweetener. Food Chem., 2021, 359: 129938.                          

[7] Zhang, B., Bian, L.Y., Huang, P.Y., Zhao, L., Chen, Y.J. *, Wu, X.R.* Efficient whole-cell biosynthesis of L-gulose by coupling mannitol-1-dehydrogenase with NADH oxidase. Enzyme Microb. Tech., 2021, 148: 109815.  

[8] Zhao, L.^#, Wang, Y. ^#, Li, Z.L., Wang, X.N., Chen, Y.J. *, Wu, X.R. * Enzymatic monoglucosylation of rubusoside and the structure−sweetness/taste relationship of monoglucosyl derivatives. J. Agric. Food Chem. 2020, 68, 8702-8709. 

[9] Zhang, C.C., Lu, M.L., Lin L., Huang, Z.J., Zhang, R.G., Wu, X.R.* Chen, Y.J.* Riboflavin is directly involved in N-dealkylation catalyzed by bacterial cytochrome P450 monooxygenases. ChemBioChem, 2020, 21(16): 2297-2305.     

Wu, X.R.,^# Jin, L.,^# Zhang, H., Tong, R.N., Ma, M. & Chen, Y.J.* Identification of truncated form of NosP as a transcription factor to regulate the biosynthesis of nosiheptide. FASEB J. 2018, 32(1): 453-465.